Intended use
Nutrient Agar is used as a general-purpose medium for the cultivation of less fastidious microorganisms and can be
enriched with blood or other biological fluids.
Composition**
Peptone: 5.000 Gms / Litre
Sodium chloride: 5.000 Gms / Litre
HM peptone B#: 1.500 Gms / Litre
Yeast extract: 1.500 Gms / Litre
Agar: 15.000 Gms / Litre
Final pH ( at 25°C): 7.4±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 28.0 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. If desired, the medium can be enriched with 5-10% blood or other biological fluids. Mix well and pour into sterile Petri plates.
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