Listeria Identification Agar Base (PALCAM)
Intended use
Recommended for selective isolation and identification of Listeria species from clinical and non-clinical samples.
Ingredients (Gms / Litre)
Peptone: 23.000
Starch: 1.000
Sodium chloride: 5.000
Mannitol: 10.000
Ammonium ferric citrate: 0.500
Esculin: 0.800
Dextrose (Glucose): 0.500
Lithium chloride: 15.000
Phenol red: 0.080
Agar: 13.000
Final pH: ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 34.44 grams in 500 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C and aseptically add rehydrated contents of 1 vial of Listeria Selective Supplement (PALCAM) (FD061). Mix well and pour into sterile Petri plates.
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