Lactobacillus MRS Broth (MRS Broth)
Ingredients (Gms / Litre)
Protease peptone: 10.000
HM Peptone B# 10.000
Yeast extract: 5.000
Dextrose(Glucose) 20.000
Polysorbate 80 (Tween 80) 1.000
Ammonium citrate 2.000
Sodium acetate 5.000
Magnesium sulphate 0.100
Manganese sulphate 0.050
Dipotassium hydrogen phosphate 2.000
Final pH ( at 25°C) 6.5±0.2
Directions
Suspend 55.15 grams in 1000 ml purified/distilled water. Heat if necessary to dissolve the medium completely. Distribute in tubes, bottles, or flasks as desired and sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
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