Himedia Lactobacillus MRS Agar, 500gm Brand: HiMedia

Lactobacillus MRS Agar (MRS Agar)
Ingredients (Gms / Litre)

Protease peptone: 10.000
HM Peptone B #: 10.000
Yeast extract: 5.000
Dextrose (Glucose): 20.000
Tween 80 (Polysorbate 80): 1.000
Ammonium citrate: 2.000
Sodium acetate: 5.000
Magnesium sulphate: 0.100
Manganese sulphate: 0.050
Dipotassium hydrogen phosphate: 2.000
Agar: 12.000
Final pH ( at 25°C): 6.5±0.2
**Formula adjusted, standardized to suit performance parameters
# Equivalent to Beef extract

Directions

Suspend 67.15 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri plates.

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