Himedia Antibiotic Assay Medium (No.1), 500gm Brand: HiMedia

Antibiotic Assay Medium No.1
Antibiotic Assay Medium No.1 is used for microbiological assay of ß-lactam and other antibiotics in accordance with the United
States Pharmacopoeia.

Ingredients (Gms / Litre)

Peptone: 6.000
Pancreatic digest of casein: 4.000
Yeast extract: 3.000
Beef extract: 1.500
Dextrose: 1.000
Agar: 15.000
pH after sterilization: 6.6±0.1
**Formula adjusted, standardized to suit performance parameters

Directions

Suspend 30.5 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Advice: Recommended for the microbiological assay of Bacitracin, Nafcillin, Cephalothin, Cephaperin, Cloxacillin,
Novobiocin, Penicillin-G

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